Journal of Multidisciplinary Dental Research
DOI: 10.38138/JMDR/v12.1.25.40
Volume: 12, Issue: 1, Pages: 1-4
Original Article
E Narendra Babu1*, Moksha Nayak2, T K Krishnameera1
1Postgraduate, Department of Conservative Dentistry and Endodontics, KVG Dental college and Hospital, Sullia, Dakshina Kannada, Karnataka 574327, India
2Professor and Principal, Department of Conservative Dentistry and Endodontics, KVG Dental college and Hospital, Sullia, Dakshina Kannada, Karnataka 574327, India
*Corresponding Author
Email: [email protected]
Received Date:12 September 2025, Accepted Date:01 December 2025, Published Date:09 July 2026
Aim of the study is to evaluate and compare the effect of grape seed extract (GSE) and amla extract (Phyllanthus emblica) as natural matrix metalloproteinases (MMP) inhibitors on microtensile bond strength (µTBS) of composite resin to dentin. Thirty human mandibular premolars were assigned equally to three groups: GSE-treated, Amla extract-treated, and control (distilled water). Following 37% phosphoric acid etching, the respective treatments were applied for 60 s. Teeth were restored using a universal adhesive (Tetric NBond) and composite (Tetric NCeram). Specimens were sectioned into beams (1 × 1mm) and subjected to µTBS testing at 0.5 mm/min. Statistical analysis included one-way ANOVA and Bonferroni post-hoc tests (α = 0.05). The GSE group demonstrated significantly higher mean µTBS (≈ 35 MPa) than both the Amla group (≈ 28 MPa) and control (≈ 20 MPa) (p < 0.05). Amla-treated specimens also showed a statistically significant improvement over control. Both grape seed and amla extracts enhance µTBS compared to untreated dentin, with grape seed extract showing superior efficacy. These results support the potential clinical application of natural MMP inhibitors to improve bond durability.
Keywords: Dentin biomodification; Grape seed extract; Amla extract; Microtensile bond strength; MMP inhibitors
1. Mazzoni A, Angeloni V, Sartori N, Duarte S Jr, Maravic T, Tjäderhane L, et al. Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time. Journal of Dental Research. 2017; 96 (8). Available from: https://doi.org/10.1177/0022034517708312
2. Mazzoni A, Nascimento FD, Carrilho M, Tersariol I, Papa V, Tjäderhane L, et al. MMP Activity in the Hybrid Layer Detected with in situ Zymography. Journal of Dental Research. 2012; 91 (5). Available from: https://doi.org/10.1177/0022034512439210
3. Heshmat H, Jahangiri MH, Hoorizad Ganjkar M, Emami Arjomand M, Kharazifard MJ. Effect of Chlorhexidine on Micro-Shear Bond Strength of Two Adhesive Systems After Aging. Journal of Islamic Dental Association of IRAN. 2018; 30 (1). Available from: https://doi.org/10.30699/jisdreir.30.1.09
4. Hass V, Luque-Martinez IV, Gutierrez MF, Moreira CG, Gotti VB, Feitosa VP, et al. Collagen cross-linkers on dentin bonding: Stability of the adhesive interfaces, degree of conversion of the adhesive, cytotoxicity and in situ MMP inhibition. Dental Materials. 2016; 32 (6). Available from: https://doi.org/10.1016/j.dental.2016.03.008
5. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. Collagen Degradation by Host-derived Enzymes during Aging. Journal of Dental Research. 2004; 83 (3). Available from: https://doi.org/10.1177/154405910408300306
6. Fawzy AS, Priyadarshini BM, Selvan ST, Lu TB, Neo J. Proanthocyanidins-Loaded Nanoparticles Enhance Dentin Degradation Resistance. Journal of Dental Research. 2017; 96 (7). Available from: https://doi.org/10.1177/0022034517691757
7. de Macedo FA, Souza NO, Lemos MV, De-Paula DM, Santiago SL, Feitosa VP. Dentin bonding and physicochemical properties of adhesives incorporated with epigallocatechin-3-gallate. Odontology. 2019; 107 (1). Available from: https://doi.org/10.1007/s10266-018-0367-0
8. Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjäderhane L, et al. In vivo Preservation of the Hybrid Layer by Chlorhexidine. Journal of Dental Research. 2007; 86 (6). Available from: https://doi.org/10.1177/154405910708600608
9. Breschi L, Martin P, Mazzoni A, Nato F, Carrilho M, Tjäderhane L, et al. Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. Dental Materials. 2010; 26 (6). Available from: https://doi.org/10.1016/j.dental.2010.02.007
10. Jain PK, Das D. Pharmacognostic comparison of Bacopa monnieri, Cyperus rotundus and Emblica officinalis. Innovare Journal of Ayurvedic Sciences. 2016;4:16–26. https://journals.innovareacademics.in/index.php/ijas/article/view/14238
11. Fujii T, Wakaizumi M, Ikami T, Saito M. Amla (Emblica officinalis Gaertn.) extract promotes procollagen production and inhibits matrix metalloproteinase-1 in human skin fibroblasts. Journal of Ethnopharmacology. 2008; 119 (1). Available from: https://doi.org/10.1016/j.jep.2008.05.039
12. Abraham S, Kumar MS, Sehgal PK, Nitish S, Jayakumar ND. Evaluation of the Inhibitory Effect of Triphala on PMN‐Type Matrix Metalloproteinase (MMP‐9). Journal of Periodontology. 2005; 76 (4). Available from: https://doi.org/10.1902/jop.2005.76.4.497
13. Bedran-Russo AK, Castellan CS, Shinohara MS, Hassan L, Antunes A. Characterization of biomodified dentin matrices for potential preventive and reparative therapies. Acta Biomaterialia. 2011; 7 (4). Available from: https://doi.org/10.1016/j.actbio.2010.12.013
14. Gupta P. Natural Products as Inhibitors of Matrix Metalloproteinases. Natural Products Chemistry & Research. 2016; 04 (01). Available from: https://doi.org/10.4172/2329-6836.1000e114
© 2026 Published by International Dental Educationists’ Association (IDEA). This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/)
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