• P-ISSN 2277-3525 E-ISSN 2582-7901

Journal of Multidisciplinary
Dental Research

Article

Journal of Multidisciplinary Dental Research

DOI: 10.38138/JMDR/v8i2.22.29

Volume: 8, Issue: 2, Pages: 57-60

Original Article

Morphological Study on Development of Vascular Endothelium in Chick Embryo

Yaiko Hara​ ✉ 1

1 Department of Anatomy, Tsurumi University School of Dental Medicine, Yokohama, 230-8501, Japan

Corresponding author ✉: [email protected]

Received Date:05 July 2022, Accepted Date:28 July 2022, Published Date:27 December 2022

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The vascular endothelium and hematopoietic stem cells are believed to derive from common progenitor cells called hemangioblasts. However, morphological study on the development of vascular endothelial cell has been scarce. The present study is intended to provide more morphological explanation of the differentiation process of the hemangioblast. We performed histological observation, using 10 four-day-old chick embryos. After fixation with 4% concentration paraformaldehyde, hematoxylin eosin staining, toluidine blue staining and immunostaining were performed in that order for observation of morphological changes of the detected cells. Blood corpuscles were aggregated in the dorsal aorta and the heart (that had been observed to beat in its preparation). Some red blood cells showed immunoreactivity to both anti-Flk-1(VEGFR2) and anti-CD31 antibody. Additionally, some blood cells had small vacuoles in the cytoplasm, and they fused to form one larger vacuole ultimately a balloon-like shape that was connected to endothelial cells. These cells were present in close proximity to the vascular endothelium. We reveal that some of the early embryonic blood cells differentiate into vascular endothelial cells.

Keywords: Vasculogenesis, Angiogenesis, Hemangioblasts, Vascular endothelial cells

References

  1. Wang X, Xiong JW. Vascular endothelial cell development and underlying mechanisms. Yi Chuan. 2012;34(9):1114–1122. Available from: https://pubmed.ncbi.nlm.nih.gov/23017452/
  2. Qiu J, Hirschi KK. Endothelial Cell Development and Its Application to Regenerative Medicine. Circulation Research. 2019;125(4):489–501. Available from: https://doi.org/10.1161/circresaha.119.311405
  4. Risau W. Mechanisms of angiogenesis. Nature. 1997;386(6626):671–674. Available from: https://doi.org/10.1038/386671a0
  5. Folkman J, D'amore PA. Blood Vessel Formation: What Is Its Molecular Basis? Cell. 1996;87(7):1153–1155. Available from: https://doi.org/10.1016/s0092-8674(00)81810-3
  6. Nardi GM, Ferrara E, Converti I, Cesarano F, Scacco S, Grassi R, et al. Does Diabetes Induce the Vascular Endothelial Growth Factor (VEGF) Expression in Periodontal Tissues? A Systematic Review. International Journal of Environmental Research and Public Health. 2020;17(8):2765. Available from: https://doi.org/10.3390/ijerph17082765
  8. Johnstone S, Logan RM. Expression of vascular endothelial growth factor (VEGF) in normal oral mucosa, oral dysplasia and oral squamous cell carcinoma. International Journal of Oral and Maxillofacial Surgery. 2007;36(3):263–266. Available from: https://doi.org/10.1016/j.ijom.2006.09.017
  9. Weng W, Sukowati WE, Sheng G. On Hemangioblasts in Chicken. PLoS One. 2007;2(11). Available from: https://doi.org/10.1371/journal.pone.0001228
  10. Park C, Ma YD, Choi K. Evidence for the Hemangioblast. Experimental Hematology. 2005;33(9):965–970. Available from: https://doi.org/10.1016/j.exphem.2005.06.003
  11. Hara Y, Wake K, Inoue K, Kuroda N, Sato A, Inamatsu M, et al. Development of the Heart Endocardium at an Early Stage of Chick Embryos Evaluated at Light- and Electron-Microscopic Levels. The Anatomical Record. 2016;299(8):1080–1089. Available from: https://doi.org/10.1002/ar.23372
  12. Ratajska A, Czarnowska E, Kołodzińska A, Kluzek W, Leśniak W. Vasculogenesis of the embryonic heart: Origin of blood island-like structures. The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology. 2006;288A(3):223–232. Available from: https://doi.org/10.1002/ar.a.20311
  13. Milgrom-Hoffman M, Harrelson Z, Ferrara N, Zelzer E, Evans SM, Tzahor E. The heart endocardium is derived from vascular endothelial progenitors. Development. 2011;138(21):4777–4787. Available from: https://doi.org/10.1242/dev.061192
  14. Hamburger V, Hamilton HL. A series of normal stages in the development of the chick embryo. Developmental Dynamics. 1992;195(4):231–272. Available from: https://doi.org/10.1002/aja.1001950404
  15. Sato T, Kaneko M, Hama A, Kusakari T, Fujieda H. Expression of class II MHC molecules in the rat pineal gland during development and effects of treatment with carbon tetrachloride. Cell and Tissue Research. 1996;284(1):65–76. Available from: https://doi.org/10.1007/s004410050567
  16. Adamo L, García-Cardeña G. The vascular origin of hematopoietic cells. Developmental Biology. 2012;362(1):1–10. Available from: https://doi.org/10.1016/j.ydbio.2011.09.008
  17. Jośsko J, Gwóźdź B, Jedrzejowska-Szypułka H, Hendryk S. Vascular endothelial growth factor (VEGF) and its effect on angiogenesis. Med Sci Monit. 2000;6(5):1047–1052. Available from: https://pubmed.ncbi.nlm.nih.gov/11208453/
  18. Herbert SP, Stainier DYR. Molecular control of endothelial cell behaviour during blood vessel morphogenesis. Nature Reviews Molecular Cell Biology. 2011;12(9):551–564. Available from: https://doi.org/10.1038/nrm3176
  19. Hervé MA, Meduri G, Petit FG, Domet TS, Lazennec G, Mourah S, et al. Regulation of the vascular endothelial growth factor (VEGF) receptor Flk-1/KDR by estradiol through VEGF in uterus. J Endocrinol. 2006;188(1):91–99. Available from: https://doi.org/10.1677/joe.1.06184
  20. Lertkiatmongkol P, Liao D, Mei H, Hu Y, Newman PJ. Endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31) Current Opinion in Hematology. 2016;23(3):253–259. Available from: https://doi.org/10.1097/moh.0000000000000239

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© 2022 Published by International Dental Educationists’ Association (IDEA). This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/)

  • 30 December 2022
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