• P-ISSN 2277-3525 E-ISSN 2582-7901

Journal of Multidisciplinary
Dental Research


Journal of Multidisciplinary Dental Research

Volume: 8, Issue: 2, Pages: 50-56

Original Article

Gingival Biotype Assessment in Association with Age, Gender and Dental Arch Location using a Novel Approach

Received Date:04 November 2022, Accepted Date:01 December 2022, Published Date:26 December 2022


To assess the gingival biotypes in a sample of periodontally healthy volunteers and to correlate their prevalence in accordance with age, gender and dental arch location using Hu-friedy Colorvue® Biotype probe. A total of ninety subjects with age groups of 16-34 years (22 males, 23 females) and 35-54 years (23 males, 22 females) were selected for this study. Gingival biotype was assessed in maxillary and mandibular anteriors and was differentiated into thin, medium, thick and very thick biotype using Colorvue® Biotype probe. Collected data was statistically analyzed using chi-square test. The association of age, gender and gingival biotype was not significant in relation to 31,32,33,41,42 and 43 but significant in relation to 11,12,13,21,22 and 23. Study showed that thick biotype decreased with advancing age. Medium and thicker gingival biotype were more prevalent in males. On dental arch comparison, the gingiva was found to be thicker in the maxillary arch as compared to mandibular arch. It was concluded that gingival thickness varies according to age, gender and dental arch. Colorvue® biotype probe was found to be an excellent tool for assessing gingival biotype in the most atraumatic and efficient way and thus aiding in selection of proper treatment protocol.


Key Messages: Since gingival biotype is gaining considerable attention as one of the key elements influencing aesthetic treatment outcome, assessment of gingival biotype is of paramount relevance. The Hu-friedy Colorvue® Biotype probe proves to be an excellent tool for assessing gingival biotype in the most atraumatic and efficient way.


Keywords: Gingival biotype, Colorvue biotype probe, Dental arch location


  1. Muller HPP, Eger T. Gingival phenotypes in young male adultsJournal of Clinical Periodontology1997;24(1):6571. Available from: https://doi.org/10.1111/j.1600-051x.1997.tb01186.x
  2. Rouck TD, Eghbali R, Collys K, Bruyn HD, Cosyn J. The gingival biotype revisited: transparency of the periodontal probe through the gingival margin as a method to discriminate thin from thick gingivaJournal of Clinical Periodontology2009;36(5):428433. Available from: https://doi.org/10.1111/j.1600-051x.2009.01398.x
  3. Kan JY, Morimoto T, Rungcharassaeng K, Roe P, Smith DH. Gingival biotype assessment is esthetic zone: Visual versus direct measurementInt J Periodontics Restorative Dent2010;30:237243. Available from: https://pubmed.ncbi.nlm.nih.gov/20386780/#:~:text=Prior%20to%20extraction%2C%20the%20gingival,using%20a%20tension%2Dfree%20caliper.
  4. Alves PHM, Alves TCLP, Pegoraro TA, Costa YM, Bonfante EA, Almeida ALPFD. Measurement properties of gingival biotype evaluation methodsClinical Implant Dentistry and Related Research2018;20(3):280284. Available from: https://doi.org/10.1111/cid.12583
  5. Abraham S, Deepak KT, Ambili R, Preeja C, Archana V. Gingival biotype and its clinical significance – A reviewThe Saudi Journal for Dental Research2014;5(1):37. Available from: https://doi.org/10.1016/j.ksujds.2013.06.003
  6. Memon S, Patel JR, Sethuraman R, Patel RR, Arora H. A comparative evaluation of the reliability of three methods of assessing gingival biotype in dentate subjects in different age groups: An in vivo studyThe Journal of Indian Prosthodontic Society2015;15(4):313317. Available from: https://doi.org/10.4103%2F0972-4052.171830
  7. Vandana KL, Savitha B. Thickness of gingiva in association with age, gender and dental arch locationJournal of Clinical Periodontology2005;32(7):828830. Available from: https://doi.org/10.1111/j.1600-051X.2005.00757.x
  8. Eghbali A, Rouck TD, Bruyn HD, Cosyn J. The gingival biotype assessed by experienced and inexperienced cliniciansJournal of Clinical Periodontology2009;36(11):958963. Available from: https://doi.org/10.1111/j.1600-051x.2009.01479.x
  9. Reddy RT, Vandana KV, Prakash S. Gingival biotype--a reviewIndian Journal of Dental Advancements2017;9(2):8692.
  10. Mallikarjuna DM, Shetty MS, Fernandes AK, Mallikarjuna RM, Iyer K. Gingival biotype and its importance in restorative dentistry: A pilot studyJournal of Interdisciplinary Dentistry2016;6(3):116120. Available from: https://www.jidonline.com/text.asp?2016/6/3/116/201651
  11. Kolte R, Kolte A, Mahajan A. Assessment of gingival thickness with regards to age, gender and arch locationJournal of Indian Society of Periodontology2014;18(4):478481. Available from: https://doi.org/10.4103/0972-124x.138699
  12. Agarwal V, SunnyMehrotra N, Vijay V. Gingival biotype assessment: Variations in gingival thickness with regard to age, gender, and arch locationIndian Journal of Dental Sciences2017;9(1):1215. Available from: http://www.ijds.in/text.asp?2017/9/1/12/201639
  13. Wara-aswapati N, Pitiphat W, Chandrapho N, Rattanayatikul C, Karimbux K. The thickness of palatal masticatory mucosa associated with ageJ Periodontol2001;72(10):14071412. Available from: https://doi.org/10.1902/jop.2001.72.10.1407
  14. Chang LC. The association between embrasure morphology and central papilla recessionJournal of Clinical Periodontology2007;34(5):432436. Available from: https://doi.org/10.1111/j.1600-051x.2007.01072.x
  15. Joshi A, Suragimath G, Zope SA, Ashwinirani SR, Varma SA. Comparison of Gingival Biotype between different Genders based on Measurement of Dentopapillary ComplexJ Clin Diagn Res2017;11(9):4045. Available from: https://doi.org/10.7860%2FJCDR%2F2017%2F30144.10575
  16. Manjunath RS, Rana A, Sarkar A. Gingival biotype assessment in a healthy periodontium: transgingival probing methodJ Clin Diagn Res2015;9(5):6669. Available from: https://doi.org/10.7860/jcdr/2015/13759.5956
  17. Shah R, Sowmya NK, Mehta DS. Prevalence of gingival biotype and its relationship to clinical parametersContemporary Clinical Dentistry2015;6(6):167171. Available from: https://doi.org/10.4103/0976-237x.166824


© 2022 Published by International Dental Educationists’ Association (IDEA). This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/)


Subscribe now for latest articles, news.